Community-acquired carriage and infections due to extended-spectrum beta-lactamase producing Enterobacteriaceae (ESBL-E) are increasing worldwide, resulting in increased morbidity, mortality and healthcare costs. The origins of community-acquired ESBL-E car- riage and infections remain unclear. Bean sprouts are a potential source of Enterobacteria- ceae for the community, as illustrated by outbreaks of pathogenic Enterobacteriaceae in the past. The current study focuses on contamination of retail bean sprouts with ESBL-E in the Netherlands. Of 131 bean sprout samples purchased between 2013 and 2016, 25 (19%) were contaminated with ESBL-E. The detected isolates were almost exclusively Klebsiella spp. and co-resistance to other antibiotics was observed frequently. Over time there was substantial genetic diversity between isolates. On the other hand, isolates from samples closely matched in time were frequently clonally related, indicative of batch contamination. Remarkably, no Escherichia coli was found. In conclusion, bean sprouts frequently harbor ESBL-E, which is a potential source for consumers.

authors: Pepijn Huizinga1,2*, Eefje Schrauwen3, Silvia Garc ́ıa-Cobos4, Ina Willemsen1,3, Carlo Verhulst1, Alexander W. Friedrich4, Paul H. M. Savelkoul5,6, John W. Rossen4, Jan Kluytmans1,7

klik hier voor volledige artikel, gepubliceerd in PLOS ONE August 30, 2018

1 Department of Infection Control, Amphia Hospital, Breda, the Netherlands, 2 Laboratory for Medical Microbiology and Immunology, Elisabeth-TweeSteden Hospital, Tilburg, the Netherlands, 3 Academy for Technology and Environmental Health, Avans University of Applied Sciences, Breda, the Netherlands,
4 University of Groningen, University Medical Center Groningen, Department of Medical Microbiology, Groningen, the Netherlands, 5 Maastricht University Medical Centre, Caphri School for Public Health and PrimaryCare,DepartmentofMedicalMicrobiology,Maastricht,theNetherlands,6 AmsterdamUniversity Medical Center, Vrije Universiteit Amsterdam, Department of Medical Microbiology & Infection Control, Amsterdam, the Netherlands, 7 Julius Center for Health Sciences and Primary Care, University Medical Center Utrecht, Utrecht University, Utrecht, the Netherlands

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